Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
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چکیده
منابع مشابه
Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis.
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and...
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Karen W. Hoover1 and Justin D. Radolf2 1Division of Sexually Transmitted Disease Prevention, National Center for HIV/AIDS, Viral Hepatitis, Sexually Transmitted Disease, and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia; and 2Departments of Medicine, Pediatrics, Immunology, and Genetics and Developmental Biology, University of Connecticut Health Center, F...
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A primary means of detecting and identifying specific proteins in cellular extracts is by Western blot analysis (4,13). Quantitation of a protein of interest is generally accomplished using an enzyme-linked immunosorbent assay (ELISA; References 8, 9 and 11) or radioimmunoassay (4,8). When ELISA or radioimmunoassay procedures for the protein of interest are unavailable, densitometric analysis o...
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Chlamydia pneumoniae is a common cause of respiratory infection. It has also been shown to be associated with coronary heart disease. Two proteins that have been reported to be recognized frequently during human infection are proteins having molecular masses of 43 and 53 kDa. In order to develop a useful alternative serological test to the microimmunofluorescence (micro-IF) assay, recombinant 4...
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ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 2004
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2004000700002